تریس هیدروکلراید

Tris Hydrochloride is a high-purity buffering compound widely used in pharmaceutical formulations, biochemical research, and analytical laboratories for precise pH control in aqueous systems, particularly within the physiological range.

🏭⚗️ Production
Prepared by controlled neutralization of high-purity Tris Base (Tris(hydroxymethyl)aminomethane) with hydrochloric acid to obtain the hydrochloride salt. The resulting crystalline product is purified, dried, and tested for assay, pH, chloride content, and heavy metal impurities to meet analytical or pharmaceutical-grade specifications (USP, BP, or AR grade). Supplied as a white, crystalline, odorless solid suitable for critical biological and analytical applications.

🔬 Properties
A white to off-white crystalline powder, freely soluble in water, forming clear, colorless solutions. Exhibits strong buffering capacity in the pH range of 7.0–9.0, with a typical pKa around 8.1 at 25 °C. Highly stable under standard laboratory conditions and compatible with a wide variety of biological and chemical reagents. Non-volatile, non-hygroscopic, and maintains stability when stored in tightly closed containers away from moisture and direct sunlight.

🧪 Applications
Widely used in the preparation of biological buffers and solutions such as Tris-HCl buffer, TBE (Tris-Borate-EDTA), and TAE (Tris-Acetate-EDTA) buffers essential for electrophoresis, protein purification, enzyme assays, and nucleic acid extraction. Plays a critical role in maintaining stable pH in diagnostic reagents, culture media, and pharmaceutical formulations. Frequently paired with Tris Base to achieve precise pH adjustments in biochemical and analytical systems.

⚠️ Safety
Classified as low toxicity and generally safe for laboratory and pharmaceutical use. May cause mild irritation to eyes, skin, and respiratory tract upon direct contact or inhalation of dust. Use appropriate personal protective equipment including gloves, goggles, and lab coat. Avoid inhalation of fine particulates and contact with strong oxidizers. Store in a cool, dry, well-ventilated area in tightly sealed containers. Dispose of material and solutions according to laboratory and environmental safety regulations.